Nummer

040-EN

Abschnitt

General Section

Anwendung

Kurzfassung

1-methyl-3-octyloxymethylimidazolium tetrafluoroborate proved to be a very good tissue fixative. Compared to fixation with formalin it resulted in comparable quality of preparations.

Substituierte Stoffe

Alternative Stoffe

Verlässlichkeit der Informationen

Internet-Informationen: Daten stammen aus einem Internet-Dokument und nur eine grundlegende Bewertung konnte durchgeführt werden

Grund für die Substitution

CMR
skin/respiratory sensitizing

Gefahrstoffbeurteilung

Substance to be substituted: Formaldehyde may cause cancer (cat. 1B), according to Annex VI of Regulation (EC) No 1272/2008 (CLP Regulation). It fulfils the criteria to be listed in the Substance Database according to SUBSPORTplus Screening Criteria (SDSC). Alternative substance: 1-Methyl-3-octyloxymethylimidazolium tetrafluoroborate is not classified according to the CLP and has no harmonised classification according to Annex VI of Regulation (EC) No 1272/2008 (CLP Regulation). » Check the Substance Database according to SUBSPORTplus Screening Criteria (SDSC)

Beschreibung der Substitution

In this study 1-methyl-3-octyloxymethylimidazolium tetrafluoroborate proved to be a very good tissue fixative. Compared to fixation with formalin it resulted in comparable quality of preparations. Several Ionic Liquids (IL) were tested to determine which IL was the best for tissue preservation and thereby suitable for histopathological diagnosis. 10% buffered formalin was used as a control. Some of the tested ILs did not preserve the tissue or the tissue did not perform well in staining. According to the study 1-methyl-3-octyloxymethylimidazolium tetrafluoroborate is non-volatile, non-flammable, non-explosive and therefore represents a reduction in the safety hazards in pathology laboratories. It proved to be reactive in immunohistochemistry and histology in a similar ways as tissues preserved in 10% buffered formalin. This ionic liquid preparations even had a much more intense staining. The study confirms the suitability of 1-methyl-3-octyloxymethylimidazolium tetrafluoroborate as fixative in histopathological procedures, eliminating the necessity of using formalin. The 1-methyl-3-octyloxymethylimidazolium tetrafluoroborate preparations even had a much more intense staining.

Bewertung des Falls/der Substitution

The search for a substitute for formaldehyde in tissue fixation is very intensive. Formaldehyde is included in the database of hazardous substances according to SUBSPORT screening criteria (SDSC). 1-methyl-3-octyloxymethylimidazolium tetrafluoroborate is not and the substance is not classified, and seems to be a better alternative.

Zeitpunkt und Ort der Umsetzung

2003 in Poland

Unternehmen, das die Alternative anwendet

University of Medical Scienses in Poznan, Poland

Art der Informationsquelle

Forschung

Art der Veröffentlichung und Verfügbarkeit

Only abstract freely available

Information zur Quelle oder Publikation: Author, Firma, Institut, Zeitpunkt der Veröffentlichung

Hazardous chemicals can be substituted By: Rikke Lethare and Christian Ege The Danish Ecological Council, February 2006. Book, 100 pages, illustrated in colours. The Danish Ecological Council https://www.ecocouncil.dk/

Publikationsquelle

Art und Verfügbarkeit der Veröffentlichung

http://www.sciencedirect.com/science/article/pii/S0065128105000334

Datum der Überprüfung

11. Dezember 2020