Case story database

Search for Successfully Implemented Alternatives

You can use the free text search function to find information in the case story database. Most suitable are: substance name and other substance identifier (EC- and CAS-number). You can refine your search after first entry for sector-, function- and process-categories. The case studies are meant to serve as source of inspiration and ideas for substitution. The alternatives are constantly being developed further. We therefore recommend that you to check in each individual case whether an alternative is suitable for your applications and purposes. If you are aware of any other relevant information that is not mentioned in the current case story, please contact SUBSPORTplus.

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Substitution of sulfochromatic mixtures

This document proposes and analyses different alternatives to sulfochromatic mixtures (chrome VI) in the cleaning of laboratory instruments. The full text is only available in French.

Substitution von Formamid durch Natriumhydroxid und Ethanol bei der Denaturierung von DNA zur Vorbereitung für die Fluoreszenz-in-situ-Hybridisierung (FISH).

Formamid wird verwendet, um DNA für die Fluoreszenz-in-situ-Hybridisierung (FISH) zu denaturieren. Die Aufgabe des Formamid ist es, die in der Probe enthaltene DNA in die Einzelstränge zu trennen, so dass eine Gensonde an einen Strang binden kann. Die FISH-Technik wird verwendet, um das Vorhandensein oder Fehlen bestimmter DNA-Sequenzen in Chromosomen zu lokalisieren. In den letzten 15 Jahren hat die Uniklinik Sahlgrenska die 70%-ige Formamidlösung durch 30ml einer 1,9%-igen Natriumhydroxidlösung und 70ml absolutem Ethanol (99,5%) substituiert.

Die Substitution funktioniert gut, und die Angestellten haben keine bestimmten Einschränkungen durch die Alternative festgestellt.

Substitution of a dish-washing compound containing Trisodium nitrilotriacetate (NTA salt) in a hospital

In the kitchen of the hospital Parc Taulí in Barcelona a dish-washing detergent containing trisodium nitrilotriacetate (NTA salt) was used. Trisodium nitrilotriacetate is a carcinogenic chemical and was substituted by a product of the same manufacturer that does not contain NTA salts.

Im nationalen Zentrum für Gesundheitsdaten und Seuchenschutz in Dänemark (SSI) ausgeführte Substitutionen.

SSI hat es geschafft, Borsäure, Ethidiumbromid und Ether durch Essigsäure, einen nicht-mutagenen DNA-Farbstoff und Natriumhydroxid zu substituieren. Derzeit wird an der Bewertung einer Alternative zu Trypanblau in der Zellfärbung gearbeitet.

Substitution of concentrated sodium hydroxide with sodium hydrogen carbonate for pH adjustment in pharmaceutical production.

Neutralisation of a product during a pharmaceutical production was earlier done by applying sodium hydroxide. Sodium hydroxide was substituted with the less hazardous alternative sodium hydrogen carbonate.

Enzymes in pharmaceutical productions can be used for degeneration of meat and for cleaning after greasy productions as an alternative for sodium hydroxide.

Enzymes can be used for cleaning after greasy productions. Lipase and alcalase can replace sodium hydroxide solutions for cleaning. The enzymes are gentler to the production equipment compared to sodium hydroxide, a strong base.

Alternatives to hydrazine in anticorrosion treatment for boilers

Hydrazine is used to protect boilers form corrosion by reducing the concentration of dissolved oxygen in the water and by contributing to the passivation of the metal surface of the installation. Hydrazine is a hazardous substance and alternatives have been found that give acceptable results.

Different ways to phase out halocarbons like dichloromethane in various applications.

This document describes how a large portion of the halocarbons used and manufactured in Sweden were phased out during the late 1980´s and early 1990´s. Several specific examples are mentioned concerning what chemicals were substituted and what the alternatives were. The chemicals discussed are mainly: dichloromethane, trichloroethylene, 1,1,2-Dichloro-1,2,2-trifluoroethane and 1,1,1-trichloroethane.

Elektrochemische Methode für die Aufbereitung von arsenhaltigem Trinkwasser

Arsen im Grundwasser ist sehr gefährlich und ein ernstes Problem ärmeren Gemeinden, die es als Trinkwasser verwenden. Eine elektrochemische, kosten-effiziente Methode zur Entfernung von Arsen wird vorgestellt und mit einer Methode verglichen, die aktiviertes Aluminiumoxid verwendet, das mit Natriumhydroxid regeneriert wird. Andere Methoden werden erwähnt und kurz verglichen.

Substitution of formamide with sodium hydroxide and ethanol in denaturation of DNA in the preparation of fluorescent in situ hybridization (FISH)

Formamide is used to denature DNA in Fluorescent In Situ Hybridization (FISH). The specific purpose of the formamide is to make the DNA in the sample single stranded, which allows a probe to bind into the remaining strand. The FISH technique is a method used to locate the presence or absence of specific sequences of DNA in chromosomes.

For the past 15 years the department for clinical genetics at Sahlgrenska University Hospital has substituted the 70% solution of formamide with 30 ml of 1.9% solution of sodium hydroxide and 70 ml of absolute ethanol (99,5%). The substitution works well and the staff has not noticed any specific limitations with the alternative.

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