Case story database

Search for Successfully Implemented Alternatives

You can use the free text search function to find information in the case story database. Most suitable are: substance name and other substance identifier (EC- and CAS-number). You can refine your search after first entry for sector-, function- and process-categories. The case studies are meant to serve as source of inspiration and ideas for substitution. The alternatives are constantly being developed further. We therefore recommend that you to check in each individual case whether an alternative is suitable for your applications and purposes. If you are aware of any other relevant information that is not mentioned in the current case story, please contact SUBSPORTplus.

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Substitution von Formaldehyd als Gewebefixiermittel

Formaldehyd ist eine organische Verbindung, die als Gewebefixiermittel in der Mikroskopie und Histologie verwendet wird. Das Formaldehyd wurde durch ein Produkt mit dem gewerblichen Namen Fixall-his substituiert, das Ethanol, Isopropanol und Essigsäure enthält.

Substitution of formaldehyde as a tissue fixative

Formaldehyde is an organic compound used as a tissue fixative for microscopy and histology. Formaldehyde was substituted by a product containing: ethyl alcohol, isopropyl alcohol and acetic acid under the commercial name Fixall-his.

Substitution von N,N-Dimethylformamid durch Ethanol bei der Analyse von alkalischer Phosphatase in Leukozyten

N,N-Dimethylformamid wird bei einem Verfahren verwendet, um die Aktivität des Enzyms alkalische Phosphatase in Leukozyten zu messen. N,N-Dimethylformamid wird speziell als Lösungsmittel eingesetzt, um eine Substratlösung anzusetzen und kann einfach durch Ethanol ausgetauscht werden, ohne dass Änderungen an diesem Arbeitsverfahren notwendig werden. Der einzige Unterschied ist, dass der Lösungsvorgang mit Ethanol etwas langsamer ist als mit N,N-Dimethylformamid.

Substitution of N,N-dimethylformamide with ethanol in analysis of alkaline phosphatases in leukocytes.

N,N-dimethylformamide is used in a method to measure activity of the enzyme alkaline phosphatase in leukocytes. N,N-dimethylformamide is specifically used as a solvent to prepare a substrate solution for the method and can easily be substituted with ethanol without any changes in the procedure. The only difference is that ethanol is slightly slower as a solvent than N,N-dimethylformamide.

Eliminierung von Trichlorethylen in Laboratorien zur Kontrolle von Asphaltqualität

Trichlorethylen wird zur Qualitätskontrolle in Laboratorien verwendet, um den Gehalt von Bitumen in Asphaltgemischen zu bestimmen. Bitumen ist ein gefährlicher Stoff, dessen Eliminierung oder Substitution im Hinblick auf Gesundheits- und Sicherheitsaspekte eine hohe Priorität zukommt. Die Eliminierung von Trichlorethylen aus dem Prozess wird mit Hilfe eines kleinen Muffelofen erreicht, der die Asphaltgemische bei der Verdunstungstemperatur von Bitumen verbrennt. Diese Prozedur ermöglicht die Berechnung des prozentualen Bitumengehalts eines Gemisches anhand des Gewichtunterschieds.

Elimination of trichloroethylene in asphalt quality control laboratories

Trichloroethylene is used in quality control laboratories to determine the amount of bitumen in asphalt mixtures. Bitumen is a hazardous substance whose elimination or substitution by a less dangerous chemical is a health and safety priority. The elimination of trichloroethylene from the process is carried out through a small muffle furnace that burns the asphalt mix at the temperature of evaporation for bitumen. This procedure facilitates the calculation of bitumen percentage in the mix based on the weight difference.

Alternatives to 2-methoxyethanol as put forward by the European Chemicals Agency (ECHA)

This document concerns the general toxicity of 2-methoxyethanol and some common alternatives to its use in different applications. The most common substitutes for 2-methoxyethanol are: PGME, EGBE, ethylene glycol monopropyl ether (EGPE) and their acetates (PGMEA, EGBEA, EGPEA). The decision which alternative to use depends on what application 2-methoxyethanol is currently used in.

Ionic liquids for tissue fixation instead of formalin

1-methyl-3-octyloxymethylimidazolium tetrafluoroborate proved to be a very good tissue fixative. Compared to fixation with formalin it resulted in comparable quality of preparations.

Substitution of 2-mercaptoethanol with 1,4-dithiothreitol for use as reducing agent in sample preparation for electrophoresis.

This example concerns the substitution of 2-mercaptoethanol with 1,4-dithiothreitol for use as a reducing agent in sample preparations of proteins or DNA/RNA before electrophoresis.

Different ways to phase out halocarbons like dichloromethane in various applications.

This document describes how a large portion of the halocarbons used and manufactured in Sweden were phased out during the late 1980´s and early 1990´s. Several specific examples are mentioned concerning what chemicals were substituted and what the alternatives were. The chemicals discussed are mainly: dichloromethane, trichloroethylene, 1,1,2-Dichloro-1,2,2-trifluoroethane and 1,1,1-trichloroethane.

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