Case story database

Search for Successfully Implemented Alternatives

You can use the free text search function to find information in the case story database. Most suitable are: substance name and other substance identifier (EC- and CAS-number). You can refine your search after first entry for sector-, function- and process-categories. The case studies are meant to serve as source of inspiration and ideas for substitution. The alternatives are constantly being developed further. We therefore recommend that you to check in each individual case whether an alternative is suitable for your applications and purposes. If you are aware of any other relevant information that is not mentioned in the current case story, please contact SUBSPORTplus.

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Substitution von Methanol durch Ethanol bei der Fixierung von Polyacrylamidgelen für die Analyse von Proteinen und DNA/RNA .

Methanol wird in einem Fixierungsprozess verwendet, der bei der Analyse
von Proteinen und DNA/RNA in Polyacrylamidgelen erforderlich ist. Diese Fixierung ist
notwendig, um Proteine oder Nukleinsäuren in große unlösbare Aggregate
umzuwandeln, die nicht reagieren oder zerfallen können. Ethanol ist ein
gutes Substitut für Methanol, da es weniger toxisch und die Arbeit mit
dem Stoff somit sicherer ist.

Die Substitution funktioniert bei der
Western Blot Analyse und der Aufbereitung von Membranen nicht gut. Es
sollte daher nicht für PVDF-Membranen verwendet werden.

Substitution of methanol with ethanol for use in fixation of polyacrylamide gels in analysis of proteins and DNA/RNA.

Methanol is used in a fixation process required for analysis of proteins and DNA/RNA on polyacrylamide gels. Fixation is necessary for transforming the proteins or nucleic acids into large, insoluble aggregates which are unable to react or degrade. Ethanol is a good substitute for methanol since it´s less toxic and therefore safer to work with.

The substitution does not work well with Western blot analysis's and processing of membranes. It should also not be used on PVDF-membranes.

Substitution of formamide with sodium hydroxide and ethanol in denaturation of DNA in the preparation of fluorescent in situ hybridization (FISH)

Formamide is used to denature DNA in Fluorescent In Situ Hybridization (FISH). The specific purpose of the formamide is to make the DNA in the sample single stranded, which allows a probe to bind into the remaining strand. The FISH technique is a method used to locate the presence or absence of specific sequences of DNA in chromosomes.

For the past 15 years the department for clinical genetics at Sahlgrenska University Hospital has substituted the 70% solution of formamide with 30 ml of 1.9% solution of sodium hydroxide and 70 ml of absolute ethanol (99,5%). The substitution works well and the staff has not noticed any specific limitations with the alternative.

Substitution von Formamid durch Natriumhydroxid und Ethanol bei der Denaturierung von DNA zur Vorbereitung für die Fluoreszenz-in-situ-Hybridisierung (FISH).

Formamid wird verwendet, um DNA für die Fluoreszenz-in-situ-Hybridisierung (FISH) zu denaturieren. Die Aufgabe des Formamid ist es, die in der Probe enthaltene DNA in die Einzelstränge zu trennen, so dass eine Gensonde an einen Strang binden kann. Die FISH-Technik wird verwendet, um das Vorhandensein oder Fehlen bestimmter DNA-Sequenzen in Chromosomen zu lokalisieren. In den letzten 15 Jahren hat die Uniklinik Sahlgrenska die 70%-ige Formamidlösung durch 30ml einer 1,9%-igen Natriumhydroxidlösung und 70ml absolutem Ethanol (99,5%) substituiert.

Die Substitution funktioniert gut, und die Angestellten haben keine bestimmten Einschränkungen durch die Alternative festgestellt.

Substitution of 2-methoxyethanol with ethanol in double esterase analysis of blood samples.

2-Methoxyethanol is a part of two different incubation media. These incubation medium are used in double esterase analysis, a kind of blood analysis.

Substitution von 2-Methoxyethanol durch Ethanol bei einer Doppel-Esterase-Analyse von Blutproben

2-Methoxyethanol ist in zwei verschiedenen Inkubationsmedien enthalten. Diese Inkubationsmedien werden bei einer Doppel-Esterase-Analyse, einer Art von Blutanalyse, verwendet.

Comb as an alternative to Lindane in treating head lice

The LiceMeister ® Comb is a medical device for routine screening, early detection and removal of head lice (pediculosis) and their nits (eggs).The combing method is superior to conventional scalp inspection.

Bio-pitch as replacement for coal tar in the production of electrodes

Bio-pitch can be mixed with char-coal to obtain electrodes. The study presents results of tests carried out with bio-pitch electrodes obtained under various conditions from eucalyptus oil.

Extraction of Active Pharmaceutical Ingredients (API’s) and other active organic compounds with Supercritical Carbon Dioxide.

Supercritical Fluid Technology utilizing carbon dioxide is highly suitable for extraction of non-polar and low-polar small compounds. The technology allows for organic solvent free extraction at low temperatures, protecting sensitive agents from processing damage.

Improvement in biodegradability and pharmaceutical properties of cytostatics for tumour treatment.

Certain pharmaceuticals including some cytostatics are carcinogenic, mutagenic, teratogenic and fetotoxic. Cytostatics and their metabolites are excreted by patients into waste water. In laboratory testing, the frequently used isomeric anti-tumour agent Ifosfamide was shown to be non- biodegradable. It is not eliminated in municipal sewage treatment plants, and therefore passes into the effluent. Structural modified compounds like Glufosfamide are still active against tumours, and in contrast to Ifosfamide are inherently biodegradable.

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