Aqueous solution combined with ultrafiltration as an alternative to 4-methylpentan-2-one (MIBK) in purification of penicillin.

Number

088-EN

Section

General Section

Use

Sector

Manufacture of bulk, large scale chemicals (including petroleum products)
Manufacture of fine chemicals
Health services
Scientific research and development

Function

Processing aid

Process

Chemical production or refinery in closed process without likelihood of exposure or processes with equivalent containment conditions.

Product category

Processing aids such as pH-regulators, flocculants, percipitant, neutalization agents
Pharmaceuticals

Application

Production of penicillin

Abstract

4-methylpentan-2-one (MIBK) was used for purification of penicillin in a pharmaceutical production. As an alternative the company used a method, where penicillin becomes water soluble through pH adjustment and then the purification can be made by ultra filtration. In this way MIBK was totally phased out.

Substituted substances

4-Methylpentan-2-one

CAS No. 108-10-1 EC No. 203-550-1 Index No. 606-004-00-4

Chemical group

Ketones

Classification: hazard statements

H225 Highly flammable liquid and vapour
H351 Suspected of causing cancer
H332 Harmful if inhaled
H336 May cause drowsiness or dizziness
H319 Causes serious eye irritation

Other adverse effects

The substance is: 2B carcinogen (IARC) as listed in the Substance Database according to SUBSPORT Screening Criteria (SDSC).

Alternative Substances

Water

CAS No. 7732-18-5 EC No. 231-791-2 Index No.

Chemical group

Non-metal oxides

Reliability of information

Evidence of implementation: there is evidence that the solution was implemented and in use at time of publication

Reason substitution

skin/respiratory sensitizing

Hazard Assessment

Substance to be substituted: 4-methylpentan-2-one (MIBK) is possibly carcinogenic to humans (Group 2B), according to the IARC Monographs. Alternative substances: The alternative is water and an unknown pH-adjusting agent. » Check the Substance Database according to SUBSPORTplus Screening Criteria (SDSC)

Description of Substitution

In the manufacturing process of penicillin, the company previously used 4-methylpentan-2-one (methyl isobutyl ketone - MIBK) for purification. Through optimisation and systematic review of well-defined and publicly available "unit operations" for the chemical industry a new method was found. Using pH adjustment penicillin becomes soluble in water. It can thereafter be purified using ultrafiltration. There are several advantages with the new method. MIBK has several properties of concern regarding occupational health and safety. It was replaced by water. Moreover, the new method is performed in a closed system and thereby the employees do not get in contact with the product. Formerly, MIBK was regenerated to be reused. This process is also eliminated. The new method can also be used for purification of other antibiotics. A disadvantage with the new method is that it has been necessary to reengineer production equipment. This investment was, however, quickly recovered, since the new process ensures a higher yield as compared to the old one. The new method has a larger water consumption than before, but the waste water is clean, so that it can be headed directly to the sewers without prior treatment.

Case/substitution evaluation

The alternative was assessed against hazards in hazardous Substance Database according to SUBSPORT Screening Criteria (SDSC) and is not listed in the database. Information is missing for a complete evaluation. But the substitution of a carcinogenic substance is necessary.

State of implementation

Full capacity

Date and place of implementation

1990 Denmark

Type of information supplier

User

Contact

www.leo-pharma.com

Date, reviewed

December 11, 2020