Case story database

Search for Successfully Implemented Alternatives

You can use the free text search function to find information in the case story database. Most suitable are: substance name and other substance identifier (EC- and CAS-number). You can refine your search after first entry for sector-, function- and process-categories. The case studies are meant to serve as source of inspiration and ideas for substitution. The alternatives are constantly being developed further. We therefore recommend that you to check in each individual case whether an alternative is suitable for your applications and purposes. If you are aware of any other relevant information that is not mentioned in the current case story, please contact SUBSPORTplus.

In order to utilize information in the best way, please take note of the SUBSPORTplus methodology on case stories!

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Substitution von Methanol und Borax in der Goldschmuckproduktion.

Einer der größten Hersteller von Goldschmuck in Dänemark hat erfolgreich den methanol- und boraxhaltigen Zusatz Flux V für Schweißbrennstoffe substituiert. Als Alternative wird eine Aceton/Wasserstoff-Mischung eingesetzt. Abgesehen vom Vorteil der Vermeidung von Borax und Methanol hat die geringere Temperatur der Schweißflamme das Präzisionsschweißen vereinfacht.

Substituting Methanol and Borax in gold jewellery production.

One of Denmark’s largest manufactures of gold jewellery has successfully substituted the welding fuel agent Flux V, containing methanol and borax. It has been substituted with an acetone/hydrogen mixture. Apart from the benefits of eliminating boron and handling of methanol in the procedure, the lower flame temperature has made the precision welding procedure more manageable.

Substitution von Methanol durch Ethanol bei der Fixierung von Polyacrylamidgelen für die Analyse von Proteinen und DNA/RNA .

Methanol wird in einem Fixierungsprozess verwendet, der bei der Analyse
von Proteinen und DNA/RNA in Polyacrylamidgelen erforderlich ist. Diese Fixierung ist
notwendig, um Proteine oder Nukleinsäuren in große unlösbare Aggregate
umzuwandeln, die nicht reagieren oder zerfallen können. Ethanol ist ein
gutes Substitut für Methanol, da es weniger toxisch und die Arbeit mit
dem Stoff somit sicherer ist.

Die Substitution funktioniert bei der
Western Blot Analyse und der Aufbereitung von Membranen nicht gut. Es
sollte daher nicht für PVDF-Membranen verwendet werden.

Substitution of methanol with ethanol for use in fixation of polyacrylamide gels in analysis of proteins and DNA/RNA.

Methanol is used in a fixation process required for analysis of proteins and DNA/RNA on polyacrylamide gels. Fixation is necessary for transforming the proteins or nucleic acids into large, insoluble aggregates which are unable to react or degrade. Ethanol is a good substitute for methanol since it´s less toxic and therefore safer to work with.

The substitution does not work well with Western blot analysis's and processing of membranes. It should also not be used on PVDF-membranes.

SUBSPORTplus Specific Substances Alternatives Assessment – Perchloroethylene

Perchloroethylene is one of the specific substances or substance groups for which a detailed alternatives assessment has been performed by SUBSPORTplus according to a common methodology. In this document the hazard profile and identified uses of perchloroethylene can be found as well as the identification, assessment and comparison of alternatives for its use for dry cleaning.

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