Nummer
146-EN
Abschnitt
General Section
Anwendung
Sektor
Wissenschaftliche Forschung und Entwicklung
Funktion
Kältemittel
Prozess
Manuelle Tätigkeiten mit Handkontakt
Produktkategorie
Laborchemikalien
Verwendung
used in electrophoresis to make DNA visible
Kurzfassung
Methylene blue may be used as an alternative to ethidium bromide, a suspected mutagen, in staining agarose gel to reveal DNA.
Substituierte Stoffe
Ethidium bromide
CAS-Nr. 1239-45-8 EC-Nr. 214-984-6 Index-Nr. 612-278-00-6
Chemische Gruppe
Bromine compounds; nitrogen heterocycles
Einstufung: H-Sätze
H341 Suspected of causing genetic defects
H330 Fatal if inhaled
H302 Harmful if swallowed
Alternative Stoffe
Methylene blue
CAS-Nr. 61-73-4 EC-Nr. 200-515-2 Index-Nr.
Chemische Gruppe
Salts of aromatic amines
Verlässlichkeit der Informationen
Internet-Informationen: Daten stammen aus einem Internet-Dokument und nur eine grundlegende Bewertung konnte durchgeführt werden
Grund für die Substitution
CMR
Gefahrstoffbeurteilung
Substance to be substituted: Ethidium bromide is: 1A or 1B carcinogen (CLP Regulation) as listed in the Substance Database according to SUBSPORTplus Screening Criteria (SDSC). Alternative substance: Methylene (methylthioninium chloride) blue is not classified as hazardous according to the harmonised classification nor is it notified in the C&L inventory. See also Further information.
Beschreibung der Substitution
Electrophoresis of DNA requires a staining step to make the separated DNA fragments visible. The safest and easiest method is to use methylene blue solution. As methylene blue carries a positive charge, it binds to the negatively charged DNA fragments and stains them blue. Two methods may be used: with diluted or with concentrated methylene blue. The method using diluted methylene blue involves submerging the gel in methylene blue solution (0.025%) and allowing it to stand for up to 16 hours so the stain diffuses into the gel and binds with the DNA fragments.
Bewertung des Falls/der Substitution
Methylene blue is a commonly used and available chemical, whose use in revealing DNA does not involve high costs or complicated laboratory procedures. It is a good substitution especially for school laboratories.
Verfügbarkeit der Alternative
Alternative available on the market
Art der Informationsquelle
Forschung
Kontakt
https://www.southernbiological.com/ https://www.southernbiological.com/
Weitere Informationen
http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=DE&language=EN-generic&productNumber=M9140&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fsearch%3Finterface%3DAll%26term%3Dmethylene%2Bblue%26lang%3Dde%26region%3DDE%26focus%3Dproduct%26N%3D0%2B220003048%2B219853101%2B219853286%26mode%3Dmatch%2520partialmax MSDS methylene blue (methylthioninium chloride) from Sigma-Aldrich
Information zur Quelle oder Publikation: Author, Firma, Institut, Zeitpunkt der Veröffentlichung
https://www.southernbiological.com/
Publikationsquelle
Art und Verfügbarkeit der Veröffentlichung
Datum der Überprüfung
11. Dezember 2020