The presented literature documentation shows the applicability of the test methods listed in regulation EC 440/2008. The first part explains different terms and definitions of nanomaterials and the nanomaterials' classifications. Afterwards the physico-chemical properties of nanomaterials are described and the feasibility to characterise substances at nanoscale with the test methods listed in regulation 440/2008 is assessed. In the last part relevant properties of nanomaterials from the perspective of international institutions are listed and possible methods for the determination are given. Finally, it is analysed which test methods of the regulation EC 440/2008 are applicable to nanomaterials and which further properties should be investigated in the frame of the regulation of nanomaterials.