Case story database

Search for Successfully Implemented Alternatives

You can use the free text search function to find information in the case story database. Most suitable are: substance name and other substance identifier (EC- and CAS-number). You can refine your search after first entry for sector-, function- and process-categories. The case studies are meant to serve as source of inspiration and ideas for substitution. The alternatives are constantly being developed further. We therefore recommend that you to check in each individual case whether an alternative is suitable for your applications and purposes. If you are aware of any other relevant information that is not mentioned in the current case story, please contact SUBSPORTplus.

In order to utilize information in the best way, please take note of the SUBSPORTplus methodology on case stories!

*For a more precise search, use quotation marks ("example").

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Substitution von Formamid durch Natriumhydroxid und Ethanol bei der Denaturierung von DNA zur Vorbereitung für die Fluoreszenz-in-situ-Hybridisierung (FISH).

Formamid wird verwendet, um DNA für die Fluoreszenz-in-situ-Hybridisierung (FISH) zu denaturieren. Die Aufgabe des Formamid ist es, die in der Probe enthaltene DNA in die Einzelstränge zu trennen, so dass eine Gensonde an einen Strang binden kann. Die FISH-Technik wird verwendet, um das Vorhandensein oder Fehlen bestimmter DNA-Sequenzen in Chromosomen zu lokalisieren. In den letzten 15 Jahren hat die Uniklinik Sahlgrenska die 70%-ige Formamidlösung durch 30ml einer 1,9%-igen Natriumhydroxidlösung und 70ml absolutem Ethanol (99,5%) substituiert.

Die Substitution funktioniert gut, und die Angestellten haben keine bestimmten Einschränkungen durch die Alternative festgestellt.

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Substitution of dichloromethane in the analysis of pesticide residue

In analysis of pesticide residues cyclohexane, ethylacetate, tert. butyl methyl ether, diethylether and light petroleum are tried as alternatives to dichloromethane. Conclusively cyclohexane, light petroleum or tert. butyl methyl ether is recommended in the article . Of these three recommended substances, light petroleum and tert. butyl methyl ether is included in the database of hazardous substances according to SUBSPORTplusS screening criteria (SDSC).

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